Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Inhibition of histone deacetylase 3 by MiR-494 alleviates neuronal loss and improves neurological recovery in experimental stroke

doi: 10.1177/0271678x19875201

Figure Lengend Snippet: Figure 6. HDAC3 inhibition reversed the miR-494 antagomir-worsened long-term outcome. (a) Experimental protocol. Mice were subjected to 45 min ischemia followed by reperfusion for 28 days, with control, miR-494 agomir, or miR-494 antagomir administered by ICV injection immediately after ischemia and RGFP966 injected intraperitoneally immediately following reperfusion. (b) Brain tissue loss at 28 days following stroke was calculated. One-way ANOVA with Tukey–Kramer post hoc test. (c) Regional CBF was monitored using two-dimensional laser speckle imaging techniques. Representative images and quantification of CBF before, during, and after transient MCAO. (d) MBP was recorded by tail cuff plethysmography using a physiological pressure transducer coupled to a data- acquisition system. (e) Motor function recovery was detected by the balance beam test. (f) Computer printouts of superimposed swimming trajectories of mice trained in light to find an invisible platform after starting in any of the four cardinal points (north, east, south, west) at the circumference of the pool. (g) Learning and memory ability were detected by escape latency in the Morris water maze. n ¼ 9–12/group. Two-way RM ANOVA followed by the Bonferroni post hoc correction. *P < 0.05, ***P < 0.001 vs. sham group; #P < 0.05 vs. MCAO þ control group; &P < 0.05 vs. MCAO þ miR-494 antagomir group.

Article Snippet: Two-dimensional laser speckle imaging and blood pressure measurement Regional cerebral blood flow (CBF) was monitored using the laser speckle imaging technique, and mean blood pressure (MBP) was recorded by tail cuff plethysmography using a physiological pressure transducer coupled to a data-acquisition system (ADInstruments, Australia).

Techniques: Inhibition, Control, Injection, Imaging